Three‐dimensional tracking and temporal analysis of liposomal transport in live cells using bright‐field imaging
Identifieur interne : 000042 ( Main/Exploration ); précédent : 000041; suivant : 000043Three‐dimensional tracking and temporal analysis of liposomal transport in live cells using bright‐field imaging
Auteurs : Feng Hing Tsai [Taïwan] ; Lin I Tai [Taïwan] ; Yu Ing Wang [Taïwan] ; Jian Ong Xiao [Taïwan] ; Tsi Suan Hsu [Taïwan] ; Chung Hi Yang [Taïwan] ; Chau Wang Lee [Taïwan]Source :
- Microscopy Research and Technique [ 1059-910X ] ; 2011-06.
English descriptors
Abstract
Gold nanoparticles (AuNPs) confined in liposomes of diameters around 200 nm produce strong scattering signal owing to surface plasmon resonance, and therefore bright‐field optical tracking of the AuNP‐encapsulating liposomes can be conducted in living cells. Using an optical profiling technique called noninterferometric wide‐field optical profilometry and a bright‐field tracking algorithm, the polynomial‐fit Gaussian weight method, we analyze three‐dimensional (3D) motion of such liposomes in living fibroblasts. The positioning accuracy in three dimensions is nearly 20 nm. We tag the liposome membranes with fibroblast growth factor‐1 and reveal the intracellular transportation processes toward or away from the nucleus. On the basis of a temporal analysis of the intracellular 3D trajectories of AuNP‐encapsulating liposomes, we identify directed and diffusive motions in the transportation processes. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc.
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DOI: 10.1002/jemt.20943
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Using an optical profiling technique called noninterferometric wide‐field optical profilometry and a bright‐field tracking algorithm, the polynomial‐fit Gaussian weight method, we analyze three‐dimensional (3D) motion of such liposomes in living fibroblasts. The positioning accuracy in three dimensions is nearly 20 nm. We tag the liposome membranes with fibroblast growth factor‐1 and reveal the intracellular transportation processes toward or away from the nucleus. On the basis of a temporal analysis of the intracellular 3D trajectories of AuNP‐encapsulating liposomes, we identify directed and diffusive motions in the transportation processes. Microsc. Res. 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